Article 1317

Title of the article

GENETIC DIFFERENTIATION AND POLYMORPHISM  OF THE VOLGA POPULATION
OF THE IMPERIAL EAGLE (AQUILA HELIACA, FALCONIFORMES, ACCIPITRIDAE) 
ACCORDING TO A MITOCHONDRIAL DNA ANALYSIS 

Authors

Korepov Mikhail Vladimirovich, Candidate of biological sciences, associate professor, sub-department of biology and chemistry, Ulyanovsk State Pedagogical University named after I. N. Ulyanov (4 100-letiya so dnya rozhdeniya V. I. Lenina square, Ulyanovsk, Russia), korepov@list.ru
Stryukov Stanislav Andreevich, Postgraduate student, Ulyanovsk State Pedagogical University named after I. N. Ulyanov (4 100-letiya so dnya rozhdeniya V. I. Lenina square, Ulyanovsk, Russia), Stanislav_str@mail.ru
Korepova Daria Aleksandrovna, Head of the Department of Nature, Ulyanovsk Regional Museum of Nature and History (3/4 Noviy Venets boulevard, Ulyanovsk, Russia), dahafomina@list.ru
Simakov Maxim Dmitrievich, Postgraduate student, Penza State University (40 Krasnaya street, Penza, Russia), maksimakov@bk.ru
Makarov Denis Konstantinovich, Junior researcher, Research Center of Fundamental and Applied Problems of Bioecology and Biotechnology, Ulyanovsk State Pedagogical University named after I. N. Ulyanov (4 100-letiya so dnya rozhdeniya V. I. Lenina square, Ulyanovsk, Russia), dk.makarov@mail.ru
Mishchenko Andrey Vladimirovich, Candidate of biological sciences, associate professor, sub-department of geography and ecology, Ulyanovsk State Pedagogical University named after I. N. Ulyanov (4 100-letiya so dnya rozhdeniya V. I. Lenina square, Ulyanovsk, Russia), a.misch@mail.ru
Titov Sergey Vital'evich, Doctor of biological sciences, professor, head of sub-department of zoology and ecology, dean of the Faculty of Physics, Mathematics and Natural Sciences, Penza State University (40 Krasnaya street, Penza, Russia), svtitov@yandex.ru

Index UDK

598.279.23:575.2(470.4)

DOI

10.21685/2307-9150-2017-3-1

Abstract

Background. The imperial eagle (Aquila heliaca) is a rare species listed in the Red book of Russia (the species with decreasing population) (2001) and the IUCN Red list (vulnerable). In the twenty-first century there has been a significant increase in the proportion of breeding territories of imperial eagles with a previously indistinctive way of nesting for the Volga Region population. The goal of the study is to analyze genetic differentiation and polymorphism of the Volga Region population of imperial eagles.
Materials and methods. Collected from 7 areas of Ulyanovsk region, 25 samples of the imperial eagle’s (Aquila heliaca) mitochondrial DNA fragment (D-loop, 345 bp) were investigated. The DNA was extract from the adult birds’ feathers. The D-loop fragments were sequenced using a set of reagents for DNA sequencing BigDye® Terminator v3.1 Cycle Sequencing Kit in a genetic analyzer ABI PRISM 3500 (Applied Biosystems). The haplotype and nucleotide diversity of the mtDNA fragments was analyzed in the program DnaSP 4.10. Pursuing the goal of identifying the haplotypes’ origin and mutational bonds the researchers constructed median networks using the software package NetWork 5.0.0.0.
Results. 8 haplotypes were identified, including four unknown for this species. There is a low degree of genetic differentiation between the geographic nesting groups (0,4–0,7 %) and between the biotopic groups (0,5 %). The maximum genetic diversity was found in Syzran steppe (Bogdanovsky Reserve) – the location of the largest nesting group of imperial eagles in Volga region. Imperial eagles of Ulyanovsk region belong to the same polymorphic population, with its high ecological flexibility regarding selection of nesting sites.
Conclusions. The research results show that the application of mitochondrial markers in studying of genetic structures of birds’ habitats is quite efficient. The Volga Region population of the Eastern Imperial eagle has a number of specific genetic traits. Increased numbers of imperial eagles in the region is the result of adaptation of the indigenous Volga region population to a variety of habitats and unusual nesting conditions. The Volga Region population of imperial eagles is characterized by a high level of genetic diversity and a low degree of genetic differentiation.

Key words

Imperial eagle, Aquila heliaca, Volga Region population, genetic differentiation and polymorphism, mtDNA

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Дата создания: 11.01.2018 15:31
Дата обновления: 11.01.2018 16:06